Van91I (PflMI) (10 U/µL) – ER0711
| 5′ | C | C | A | N | N | N | N ↓ | N | T | G | G | 3′ | ||||
| 3′ | G | G | T | N ↑ | N | N | N | N | A | C | C | 5′ |
Thermo Scientific Van91I (PflMI) restriction enzyme recognizes CCANNNN^NTGG sites and cuts best at 37°C in R buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. Isoschizomers: AccB7I, BasI, PflMI.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
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ECOMMENDATIONS
1X Buffer R (for 100% Van91I digestion)
10 mM Tris-HCl (pH 8.5), 10 mM MgCl 2
, 100 mM KCl,
0.1 mg/mL BSA.
Incubation temperature
37°C.
Unit Definition
One unit is defined as the amount of Van91I required to
digest 1 μg of lambda DNA in 1 hour at 37°C in 50 μL of
recommended reaction buffer.
Dilution
Dilute with Dilution Buffer (#B19): 10 mM Tris-HCl
(pH 7.4 at 25°C), 100 mM KCl, 1 mM EDTA, 1 mM DTT,
0.2 mg/mL BSA and 50% glycerol.
Double Digests
Thermo Scientific Tango Buffer is provided to simplify buffer
selection for double digests. 98% of Thermo Scientific
restriction enzymes are active in a 1X or 2X concentration
of Tango
™ Buffer. Please refer to
www.thermoscientific.com/doubledigest to choose
the best buffer for your experiments.
1X Tango Buffer: 33 mM Tris-acetate (pH 7.9 at 37°C),
10 mM magnesium acetate, 66 mM potassium acetate,
0.1 mg/mL BSA
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