Thermo Fisher Scientific
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MspI (HpaII) – ER0541
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
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MssI (PmeI) – ER1341
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
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MunI (MfeI)- ER0751
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
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NdeI (10 U/µL) – 500 units – ER0581
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
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NotI (10 U/µL)- 300 units – ER0591
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
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PacI – ER2201
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
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PagI (BspHI) – ER1281
5′ T ↓ C A T G A 3′ 3′ A G T A C ↑ T 5′ Thermo Scientific PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (isoschizomers: BspHI, CciI, RcaI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion,…
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PdmI (XmnI) – ER1531
Molecular cloning•
Restriction site mapping•
Genotyping•
Southern blotting•
Restriction fragment length polymorphism (RFLP)•
SNP•
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Ppu21I (BsaAI) – ER1971
5′ Y A C ↓ G T R 3′ 3′ R T G ↑ C A Y 5′ Thermo Scientific Ppu21I (BsaAI) – ER1971 restriction enzyme recognizes YAC^GTR sites and cuts best at 30°C in its own unique buffer (isoschizomers: BsaAI, BstBAI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double…
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PstI (10 U/µL) – ER0611 – 3,000 units
Storage Buffer
PstI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C),
200 mM NaCl, 1 mM DTT, 0.1 mM EDTA,
0.15% Triton X-100, 0.2 mg/mL BSA and 50% glycerol.
Recommended Protocol for Digestion
• Add:
nuclease-free water 16 μL
10X Buffer O 2 μL
DNA (0.5-1 μg/μL) 1 μL
PstI 0.5-2 μL
• Mix gently and spin down for a few seconds.
• Incubate at 37°C for 1-16 hours.
The digestion reaction may be scaled either up or down.
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RevertAid First Strand cDNA Synthesis Kit Fermentas
• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit—all the components for the RT reaction are included
Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis
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RsaI – ER1121
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
برای قیمت تماس بگیرید
SacI – ER1132
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
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SalI (10 U/µL) – ER0641
SalI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C),
100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA
and 50% glycerol.
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SsiI (AciI) – ER1791
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
برای قیمت تماس بگیرید
