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SalI (10 U/µL) – ER0641
SalI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C),
100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA
and 50% glycerol.
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SsiI (AciI) – ER1791
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
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T4 DNA Ligase (5 U/µL)- EL0014 – Thermo Scientific
Applications
• Cloning of restriction enzyme generated DNA fragments
• Cloning of PCR products
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T7 RNA Polymerase (20 U/µL) – EP0111
Applications
Synthesis of unlabeled and labeled RNA that can be used:
• For hybridization, in vitro RNA translation
• As aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing
• In studies of RNA secondary structure and RNA-protein interactions, RNA splicing
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Tango-Puffer (10X) – BY5 – 5 x 1.0 mL
Der Thermo Scientific 10X Puffer Y gewährleistet optimale Reaktionsbedingungen für Restriktionsenzyme und wird mit BSA vorgemischt, um die Stabilität zu erhöhen. Unser Fünf-Puffer-System gewährleistet optimale Reaktionsbedingungen für jedes Restriktionsenzym. Dieses System besteht aus 10 x B(Blau)-, G(Grün)-, O(Orange)-, R(Rot)- und Tango(Gelb)-Puffern. Alle Restriktionsenzyme werden in farblich gekennzeichneten Röhrchen geliefert, um den empfohlenen Reaktionspuffer anzugeben. Der…
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TaqI – ER0671
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
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Tru1I (MseI) (10 U/µL)
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
برای قیمت تماس بگیرید
Van91I (PflMI) (10 U/µL) – ER0711
ECOMMENDATIONS
1X Buffer R (for 100% Van91I digestion)
10 mM Tris-HCl (pH 8.5), 10 mM MgCl 2
, 100 mM KCl,
0.1 mg/mL BSA.
Incubation temperature
37°C.
Unit Definition
One unit is defined as the amount of Van91I required to
digest 1 μg of lambda DNA in 1 hour at 37°C in 50 μL of
recommended reaction buffer.
Dilution
Dilute with Dilution Buffer (#B19): 10 mM Tris-HCl
(pH 7.4 at 25°C), 100 mM KCl, 1 mM EDTA, 1 mM DTT,
0.2 mg/mL BSA and 50% glycerol.
Double Digests
Thermo Scientific Tango Buffer is provided to simplify buffer
selection for double digests. 98% of Thermo Scientific
restriction enzymes are active in a 1X or 2X concentration
of Tango
™ Buffer. Please refer to
www.thermoscientific.com/doubledigest to choose
the best buffer for your experiments.
1X Tango Buffer: 33 mM Tris-acetate (pH 7.9 at 37°C),
10 mM magnesium acetate, 66 mM potassium acetate,
0.1 mg/mL BSA
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XbaI (10 U/µL) – 1,500 units – ER0681
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
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XhoI – ER0691
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
برای قیمت تماس بگیرید
