Recommended Protocol for Digestion
 Add:
nuclease-free water 16 μL
10X Buffer Tango 2 μL
DNA (0.5-1 μg/μL) 1 μL
BspLI 0.5-2 μL
 Mix gently and spin down for a few seconds.
 Incubate at 37°C for 1-16 hours.
The digestion reaction may be scaled either up or down.
Recommended Protocol for Digestion of PCR Products
Directly after Amplification
 Add:
PCR reaction mixture 10 μL (~0.1-0.5 μg of DNA)
nuclease-free water 18 μL
10X Buffer Tango 2 μL
BspLI 1-2 μL
 Mix gently and spin down for a few seconds.
 Incubate at 37°C for 1-16 hours.