آنزیم ها
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NcoI (10 U/µL) – ER0571
Superior quality—stringent quality control and industry leading manufacturing process
Convenient color-coded Five Buffer System
Includes universal Tango buffer for double-digestions
BSA premixed in reaction buffers
Wide selection of restriction endonuclease specificities
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NdeI (10 U/µL) – 500 units – ER0581
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
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NotI (10 U/µL)- 300 units – ER0591
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
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PacI – ER2201
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
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PagI (BspHI) – ER1281
5′ T ↓ C A T G A 3′ 3′ A G T A C ↑ T 5′ Thermo Scientific PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (isoschizomers: BspHI, CciI, RcaI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion,…
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PdmI (XmnI) – ER1531
Molecular cloning•
Restriction site mapping•
Genotyping•
Southern blotting•
Restriction fragment length polymorphism (RFLP)•
SNP•
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PfeI (TfiI) (10 U/µL) (500 Units) – ER1781
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
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Phusion DNA polymerase
این آنزیم پروتئینی نوترکیب با درجه خلوص بالا می باشد که پلیمریزاسیون نوکلئوتیدها به DNA دوبلکس را در جهت ´3 <´5 کاتالیز می کند. در نتیجه منجر به تولید محصولات PCR با انتهای صاف بدون اورهنگ´3-dA می شود.
Pfu بر خلاف آنزیم Taq دارای فعالیت تصحیح کنندگی´3 >´5 می باشد. میزان خطای آنزیم پی اف یو پلیمراز 1 در 1.3 میلیون جفت باز به ازای تشکیل قطعه 1 کیلو باز در PCR می باشد، در حالیکه میزان خطای آنزیم تک پلیمراز 1 در 9000 نوکلئوتید است. همچنین پایداری این آنزیم در برابر حرارت بیشتر از آنزیم تک است. اگرچه این آنزیم سرعت کمتری نسبت به آنزیم تک دارد و به حدود 2 دقیقه زمان در هر چرخه به ازای هر 1 کیلو باز در دمای 72 درجه سانتیگراد نیاز دارد. به علت میزان دقت و فیدلیتی بالاتر آنزیم Pfu DNA Polymerase در مقایسه با آنزیم تک در PCR، این آنزیم بیش از 10 برابر مطمئن تر می باشد.
Price range: 800,000 تومان through 1,550,000 تومان
Phusion High-Fidelity DNA Polymerase – F530S
High fidelity (52X Taq)
Fast PCR due to short extension times (15-30 s/kb)
Robust performance, minimal optimization needed
High yields of PCR products with minimal enzyme amounts
Available as a Green buffer format for direct loading of PCR products on gels
12,650,000 تومان
Ppu21I (BsaAI) – ER1971
5′ Y A C ↓ G T R 3′ 3′ R T G ↑ C A Y 5′ Thermo Scientific Ppu21I (BsaAI) – ER1971 restriction enzyme recognizes YAC^GTR sites and cuts best at 30°C in its own unique buffer (isoschizomers: BsaAI, BstBAI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double…
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Proteinase K- 20mg/ml – 1ml
Proteinase K from Parengyodontium album (Tritirachium album) is a subtilisinrelated serine protease. It is a broad spectrum endopeptidase with very high specific activity. It is widely used for digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising integrity of isolated DNA or RNA. Proteinase K is active under wide range of…
675,000 تومان
PstI (10 U/µL) – ER0611 – 3,000 units
Storage Buffer
PstI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C),
200 mM NaCl, 1 mM DTT, 0.1 mM EDTA,
0.15% Triton X-100, 0.2 mg/mL BSA and 50% glycerol.
Recommended Protocol for Digestion
• Add:
nuclease-free water 16 μL
10X Buffer O 2 μL
DNA (0.5-1 μg/μL) 1 μL
PstI 0.5-2 μL
• Mix gently and spin down for a few seconds.
• Incubate at 37°C for 1-16 hours.
The digestion reaction may be scaled either up or down.
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RevertAid First Strand cDNA Synthesis Kit Fermentas
• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit—all the components for the RT reaction are included
Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis
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Ribonuclease A DNase and Protease Free 20mg/ml
The working concentration of RNase A is 1‐100μg/ml,
depending on the application.
The enzyme is active under a wide range of reaction
conditions. At low salt concentrations (to 100mM NaCl),
RNase A cleaves single‐stranded and double‐stranded RNA
as well the RNA strand in RNA‐DNA hybrids. However, at
NaCl concentrations of 0.3M or higher, RNase A specifically
cleaves single‐stranded RNA.
Applications
Plasmid and genomic DNA preparation.
Removal of RNA from recombinant protein
preparations.
Ribonuclease protection assays .
Mapping single‐base mutations in DNA or RNA.
795,000 تومان
RsaI – ER1121
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
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