ECOMMENDATIONS
1X Buffer R (for 100% Van91I digestion)
10 mM Tris-HCl (pH 8.5), 10 mM MgCl 2
, 100 mM KCl,
0.1 mg/mL BSA.
Incubation temperature
37°C.
Unit Definition
One unit is defined as the amount of Van91I required to
digest 1 μg of lambda DNA in 1 hour at 37°C in 50 μL of
recommended reaction buffer.
Dilution
Dilute with Dilution Buffer (#B19): 10 mM Tris-HCl
(pH 7.4 at 25°C), 100 mM KCl, 1 mM EDTA, 1 mM DTT,
0.2 mg/mL BSA and 50% glycerol.
Double Digests
Thermo Scientific Tango Buffer is provided to simplify buffer
selection for double digests. 98% of Thermo Scientific
restriction enzymes are active in a 1X or 2X concentration
of Tango
™ Buffer. Please refer to
www.thermoscientific.com/doubledigest to choose
the best buffer for your experiments.
1X Tango Buffer: 33 mM Tris-acetate (pH 7.9 at 37°C),
10 mM magnesium acetate, 66 mM potassium acetate,
0.1 mg/mL BSA