HhaI (10 U/µL) – ER1851

5′ G C G ↓ C 3′
3′ C ↑ G C G 5′

Thermo Scientific HhaI restriction enzyme recognizes GCG^C sites and cuts best at 37°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. Isoschizomers: AspLEI, BstHHI, CfoI, HinP1I, HspAI.