MspI (HpaII) – ER0541

5′ C ↓ C G G 3′
3′ G G C ↑ C 5′

Thermo Scientific MspI (HpaII) restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.