HindIII – ER0501

5′ A ↓ A G C T T 3′
3′ T T C G A ↑ A 5′

Thermo Scientific HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.