کیسه دیالیز 3.5 کیلو دالتن

کیسه دیالیز 3.5 کیلو دالتن

روش استفاده:

To avoid contamination, tubings should only be touched with
gloves.
The tubings are delivered dry. They are treated with glycerol as humectant to prevent
them from drying out. Due to the manufacturing process, they contain traces of heavy
metals (<50 ppm) and sulfur compounds (<0.3%). For most biochemical applications,
it is suggested to remove these compounds by boiling and washing. Several methods
have been described in the literature
1) Boiling successively (each time 15 minutes) in 5% Na2CO3, 50 mM EDTA pH 8,
and dist. water; between each step, the tubings have to be rinsed with dist. water
2) Boiling 30 minutes in 10 mM EDTA pH 7.5. Storage at 4° C in 1 mM EDTA pH 7.5
3) Heating to 70° – 80° C for 2 hours in heavy metal free water with occasional gentle
stirring. The water is decanted and this procedure is repeated 3 more times. The
tubings are stored in heavy metal free water or in water with Na-azide
It is advisable to prepare a larger stock in one of these ways and to store it in the
refrigerator. Before use, tubings have to be thoroughly washed with dist. water.
The samples are filled with a pipette into the tubings – closed at the lower end with a
clamp. Closing by knots is not recommended, as it may damage the membrane and
result in loss of material during dialysis.
As the volume of the protein solution will increase during the dialysis process due to
penetration of water, the tubing should be filled only by 2/3.
For dialysis, the tubing, closed on both ends, is placed into a sufficiently large vessel
filled with buffer and stirred on a magnetic stirrer. Depending on the concentration
gradient and the requirements of the following procedures, we recommend 1 – 3 buffer
exchanges

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