Eco47I (AvaII) (10 U/µL) – ER0311
| 5′ | G ↓ | G | W | C | C | 3′ | ||||
| 3′ | C | C | W | G ↑ | G | 5′ |
Thermo Scientific Eco47I (AvaII) (10 U/µL) – ER0311 restriction enzyme recognizes G^GWCC sites and cuts best at 37°C in R buffer Isoschizomers: AvaII, Bme18I, SinI, VpaK11BI. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.
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One unit is defined as the amount of Eco47I required to
digest 1 μg of lambda DNA in 1 hour at 37°C in 50 μL of
recommended reaction buffer.
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