Ribonuclease A DNase and Protease Free 20mg/ml
Ribonuclease A (RNase A) is an endoribonuclease that
specifically degrades single‐standarded RNA at C and U
residues. It cleaves the phosphodiester bond between the
5’‐ribose of a nucleotide and the phosphate group attached
to the 3’‐ribose of an adjacent pyrimidine nucleotide. The
resulting 2′,3’‐cyclic phosphate is hydrolyzed to the
corresponding 3’‐nucleoside phosphate.
795,000 تومان
برند: سیناکلون
کاربرد: بیوتکنولوژی, دارویی و داروسازی, صنایع غذایی, پاتولوژی, کشاورزی, کلینیکال
کد کالا:
وضعیت موجودی: موجود
ویژگی های محصول
The working concentration of RNase A is 1‐100μg/ml,
depending on the application.
The enzyme is active under a wide range of reaction
conditions. At low salt concentrations (to 100mM NaCl),
RNase A cleaves single‐stranded and double‐stranded RNA
as well the RNA strand in RNA‐DNA hybrids. However, at
NaCl concentrations of 0.3M or higher, RNase A specifically
cleaves single‐stranded RNA.
Applications
Plasmid and genomic DNA preparation.
Removal of RNA from recombinant protein
preparations.
Ribonuclease protection assays .
Mapping single‐base mutations in DNA or RNA.
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