RsaI – ER1121

5′ G T ↓ A C 3′
3′ C A ↑ T G 5′

Thermo Scientific RsaI restriction enzyme recognizes GT^AC sites and cuts best at 37°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. Isoschizomers: AfaI, CviQI, RsaNI.