TEMPase Hot Start DNA Polymerase is a chemically modified version of Ampliqon Taq DNA Polymerase and is activated by heat treatment.

A chemical moiety is attached to the enzyme at the active site, which renders the TEMPase Hot Start enzyme inactive at room temperature.
During set up and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. Once the PCR reaction reaches the optimal activation temperature, the chemical moiety is cleaved releasing the active TEMPase Hot Start DNA Polymerase. Resulting in higher specificity, sensitivity and yield compared to standard Ampliqon Taq DNA polymerase.