Thermo Fisher Scientific
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AdeI (DraIII) – ER1231
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
1 تومان
AjiI (BmgBI) – ER1941
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
0 تومان
AluI (10 U/µL) – ER0011 – 600 units
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
1 تومان
BclI (10 U/µL) – 1,000 units – ER0721
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
برای قیمت تماس بگیرید
BcuI (SpeI) – ER1251
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
برای قیمت تماس بگیرید
BpiI (BbsI) – ER1011
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
برای قیمت تماس بگیرید
BseLI (BslI) – ER1201
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
• SNP
برای قیمت تماس بگیرید
BspLI (NlaIV) (10 U/µL) – ER1151
Recommended Protocol for Digestion
Add:
nuclease-free water 16 μL
10X Buffer Tango 2 μL
DNA (0.5-1 μg/μL) 1 μL
BspLI 0.5-2 μL
Mix gently and spin down for a few seconds.
Incubate at 37°C for 1-16 hours.
The digestion reaction may be scaled either up or down.
Recommended Protocol for Digestion of PCR Products
Directly after Amplification
Add:
PCR reaction mixture 10 μL (~0.1-0.5 μg of DNA)
nuclease-free water 18 μL
10X Buffer Tango 2 μL
BspLI 1-2 μL
Mix gently and spin down for a few seconds.
Incubate at 37°C for 1-16 hours.
برای قیمت تماس بگیرید
BstXI – ER1021
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
برای قیمت تماس بگیرید
BsuRI (HaeIII) – ER0151
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
برای قیمت تماس بگیرید
DNase I, RNase-free – EN0521
Applications
• Preparation of DNA-free RNA
• Removal of template DNA following in vitro transcription
• Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
• DNA labeling by nick-translation in conjunction with DNA Polymerase I
• Studies of DNA-protein interactions by DNase I, RNase-free footprinting
• Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used
برای قیمت تماس بگیرید
DreamTaq™ Hot Start DNA Polymerase – 200 U – EP1701
Applications:
• Routine PCR
• Colony PCR
• Genotyping
• RT-PCR
• Generation of PCR products for TA cloning
برای قیمت تماس بگیرید
Eco130I (StyI) – ER0411
5′ C ↓ C W W G G 3′ 3′ G G W W C ↑ C 5′ Thermo Scientific Eco130I (StyI) restriction enzyme recognizes C^CWWGG sites and cuts best at 37°C in O buffer (Isoschizomers: BssT1I, EcoT14I, ErhI, StyI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double…
برای قیمت تماس بگیرید
Eco47I (AvaII) (10 U/µL) – ER0311
One unit is defined as the amount of Eco47I required to
digest 1 μg of lambda DNA in 1 hour at 37°C in 50 μL of
recommended reaction buffer.
برای قیمت تماس بگیرید
Eco72I (PmlI) – ER0361
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
برای قیمت تماس بگیرید
EcoRI – ER0271
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
برای قیمت تماس بگیرید
